Neuroprotective effects of Oleiferin F on mitochondrial-mediated apoptosis in glutamate-induced HT22 cells

Jung-Soo Park, Jong-Hwa Park, Ki-Young Kim

Abstract


Neuronal disease is caused by neuronal cell damage, and a high concentration of glutamate causes neuronal cell death. The purpose of this experiment was to confirm the protective effect of Oleiferin F (OF) on glutamate-induced apoptosis in HT22 cells. Oleiferin F inhibited the death of glutamate-induced HT22 cells in a dose-dependent manner as confirmed by annexin V/propidium iodide (PI) double staining. Oleiferin F reduced disruption of the mitochondrial membrane potential (ΔΨm), p53 expression, the Bax/Bcl-2 expression ratio and mitochondrial apoptosis-inducing factor (AIF) protein release. Oleiferin F also reduced levels of the cleaved form of caspase pathway proteins (PARP, caspase-9, caspase-3), which lead to apoptosis. These results reveal the mechanism underlying the neuroprotective effect of Oleiferin F in HT22 cells. Oleiferin F may be useful in preventing or treating neurological diseases.

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Published: 2020-06-08

How to Cite this Article:

Jung-Soo Park, Jong-Hwa Park, Ki-Young Kim, Neuroprotective effects of Oleiferin F on mitochondrial-mediated apoptosis in glutamate-induced HT22 cells, Commun. Math. Biol. Neurosci., 2020 (2020), Article ID 28

Copyright © 2020 Jung-Soo Park, Jong-Hwa Park, Ki-Young Kim. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Commun. Math. Biol. Neurosci.

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